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  • br Fig Proliferation inhibition of Mel Rm cell

    2020-08-12


    Fig. 17. Proliferation inhibition of Mel-Rm cell lines by L-Dopa, [email protected], and [email protected]@L-Dopa after 24 h. The 4152-77-6 were counted by the MTT.
    There were three Groups: Group I: incubated with L-Dopa, Group II: incubated with [email protected]@L-Dopa, and Group III: incubated with [email protected]
    Fig. 16. The cell cytotoxicity of Mel-Rm cells in different treatment media.
    There were three Groups: Group I: incubated with L-
    Dopa, Group II: incubated with [email protected]@L-Dopa, and Group III: incubated with [email protected] There were control and six treatments in each group, including; control: 0.0 μM, treatment 1: 1 μM, treatment 2: 2 μM, treatment 3: 4 μM, treatment 4: 8 μM, treatment 5: 16 μM, and treatment 6: 32 μM of material in each groups, respectively. All data represented by mean ± S.E.M (p < 0.05). Significant difference be-tween treatments and control treatment in each group; *p < 0.05; one way ANOVA for repeated measures.
    Fig. 18. The effects of different treatments on the cell death on Mel-Rm cells in different treatment media. There were three Groups: Group I: incubated with L-Dopa, Group II: incubated with [email protected]@L-Dopa, and Group III: incubated with [email protected] There were control and six treatments in each group, including; control: 0.0 μM, treatment 1:1 μM, treatment 2:2 μM, treatment 3:4 μM, treatment 4:8 μM, treatment
    5:16 μM, and treatment 6:32 μM of material in each groups, respectively. All data represented by mean ± S.E.M (p < 0.05). Significant difference be-tween treatments and control treatment in each group; *P < 0.05; one way ANOVA for repeated measurements.
    Fig. 19. The effects of different treatments on the cas-pase-3 activity on Mel-Rm cells.
    There were three Groups: Group I: incubated with L-
    Dopa, Group II: incubated with [email protected]@L-Dopa, and Group III: incubated with [email protected] There were control and six treatments in each group, including; control: 0.0 μM, treatment 1: 1 μM, treatment 2: 2 μM, treatment 3: 4 μM, treatment 4: 8 μM, treatment
    5: 16 μM, and treatment 6: 32 μM of material in each groups, respectively. All data represented by mean ± S.E.M (p < 0.05). Significant difference for treatments and control treatment in each group; *P < 0.05; one way ANOVA for repeated measurements.
    Fig. 20. The effects of different treatments on the Mitochondrial membrane potential (Rhodamine-123 ab-sorbance) in Mel-Rm cells.
    There were three Groups: Group I: incubated with L-
    Dopa, Group II: incubated with [email protected]@L-Dopa, and Group III: incubated with [email protected] There were control and six treatments in each group, including; control: 0.0 μM, treatment 1: 1 μM, treatment 2: 2 μM, treatment 3: 4 μM, treatment 4: 8 μM, treatment
    5: 16 μM, and treatment 6: 32 μM of material in each groups, respectively. All data represented by mean ± S.E.M (p < 0.05). Significant difference be-tween treatments and control treatment in each group; *P < 0.05; one way ANOVA for repeated measurements.
    synergistic antitumor activity reveals the great potential for future ap-plications of this new L-Dopa release system in cancer chemotherapy. Further investigation on this topic is required.
    In the L-Dopa group (Fig. 18), the results of this experiment showed that, in treatments 1–6, the percentage of cell death was increased compared with control cells (p < 0.05). In [email protected]@L-Dopa 
    group, exposure of the cells to the different concentrations of nano-particle was increased the cell death in treatments 1–6 compared with control cells (p < 0.05). The percentage of cell death was increased in treatments 5 and 6 compared with treatments 1–3 (p < 0.05). In [email protected]@L-Dopa group, exposure of the cells to the different concentrations of nano-drug was increased the cell death in treatments 1–6 compared with intra-control cells and L-Dopa group treatments, respectively (p < 0.05).
    Fig. 21. The effects of different concentrations of L-Dopa induced apoptosis in Mel-Rm cells as identified by DNA fragmentation (TUNEL staining) (400×). Viable cell: white arrow and apoptotic cell: yellow arrow. A:control: 0.0 μM, B:treatment 1: 1 μM, C:treatment 2: 2 μM, D:treatment 3: 4 μM, E:treatment 4: 8 μM, F:treatment 5: 16 μM, and G:treatment 6: 32 μM. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    In most cases, L-Dopa and [email protected]@L-Dopa groups, eventually mediate a common apoptotic pathway through the result was obtained in case of caspase-3 activation (Fig. 19). Furthermore, in [email protected] group, Caspase3 activation in all treatments after 24 h was similar to control treatment. In the L-Dopa group: the activa-tion of caspase-3 in control cells was less than other treatments (treatments 1–6) (p < 0.05). Caspase-3 activation in treatments 1–6 were higher compared with [email protected] treatment, respectively (p < 0.05). Caspase-3 activation in treatment 1 was less in comparison with treatments 2–6, respectively (p < 0.05).